P01
A novel technology for accurate quantification of viral genome and determination of lipid nanoparticle encapsulation efficiency
S Mondal(2) N Feirer(2) I Prost(1) K Hsiao(2) M Seirup(2) T Hoang(2) M Urh(2) J J Cali(2)
1:Promega France; 2:Promega Corp
Adeno-associated viral (AAV) vectors and lipid nanoparticles (LNP) are promising delivery systems for gene therapy and vaccines, respectively. However, quantifying their effectiveness has been challenging.
The TruTiter™ Reagent is a small molecule that is excluded from encapsulated or compartmentalized macromolecular structures like viruses with an intact protein capsid, living cells, or LNPs with intact membranes. However, the molecule covalently conjugates exposed nucleic acid and prevents subsequent amplification by PCR. This property enables selective amplification of fully encapsulated nucleic acids for accurately measuring intact AAV and LNP genome titers. The reagent has been used to consistently quantify AAV genome titers, proving to be a reliable and more precise alternative to DNase. In addition, as TruTiter™ Reagent is excluded from the LNP particles, it was utilized to determine encapsulation efficiency, a property important for choosing the right type of lipid and understanding storage conditions.